Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | Mus musculus | - |
(5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | - |
? | |
arachidonate + O2 | Mus musculus C57BL/6 | - |
(5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P39654 | - |
- |
Mus musculus C57BL/6 | P39654 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
aorta | - |
Mus musculus | - |
artery | - |
Mus musculus | - |
endothelium | - |
Mus musculus | - |
monocyte | - |
Mus musculus | - |
vascular smooth muscle cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Mus musculus | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | - |
? | |
arachidonate + O2 | - |
Mus musculus C57BL/6 | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
12/15-lipoxygenase | - |
Mus musculus |
12/15-LO | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of 12/15-LO negates endothelial tight junctions disruption and monocyte adhesion caused by the high-fat diet | Mus musculus |
physiological function | 12/15-lipoxygenase plays a role in atherosclerosis. 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE), the major 12/15-LO metabolite of arachidonic acid, induces endothelial barrier permeability via Src and Pyk2-dependent zonula occluden (ZO)-2 tyrosine phosphorylation and its dissociation from the tight junction complexes. 15(S)-HETE also stimulates macrophage adhesion to the endothelial monolayer in Src and Pyk2-dependent manner. Exposure of arteries from wild-type mice to arachidonate or 15(S)-HETE leads to Src-Pyk2-dependent ZO-2 tyrosine phosphorylation, tight junction disruption, and macrophage adhesion, whereas the arteries from 12/15-LO knock-out mice are protected from these effects of arachidonate. Feeding wild-type mice with a high-fat diet induces the expression of 12/15-LO in the arteries leading to tight junction disruption and macrophage adhesion, and deletion of the 12/15-LO gene disallows these effects. 15(S)-HETE-induced endothelial tight junctions disruption promotes monocyte transmigration, 15(S)-HETE induces the dislocation of both claudin-1 and claudin-5 from ZO-2, that participates in tight junctions | Mus musculus |